絞り込み

16765

広告

High copy number and highly stable Escherichia coli-Bacillus subtilis shuttle plasmids based on pWB980.

著者 Zhao X , Xu J , Tan M , Zhen J , Shu W , Yang S , Ma Y , Zheng H , Song H
Microb Cell Fact.2020 Feb 07 ; 19(1):25.
この記事をPubMed上で見るPubMedで表示
この記事をGoogle翻訳上で見る Google翻訳で開く

スターを付ける スターを付ける     (1view , 0users)

Full Text Sources

pWB980 derived from pUB110 is a promising expression vector in Bacillus for its high copy number and high stability. However, the low transformation rate of recombinant plasmids to the wild cells limited the application of it. On the basis of pWB980, constructing an E. coli-B. subtilis shuttle plasmid could facilitate the transformation rate to Bacillus cells. Because the insertion site for E. coli replication origin sequence (ori) is not unique in pWB980, in order to investigate the best insertion site, eight shuttle plasmids (pUC980-1 ~ pUC980-8) containing all possible insertion sites and directions were constructed.
PMID: 32028973 [PubMed - in process]
印刷用ページを開く Endnote用テキストダウンロード