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Estrous cycle impacts miRNA content in extracellular vesicles that modulate bovine cumulus cell transcripts during in vitro maturation.

著者 de Ávila ACFCM , Bridi A , Andrade GM , Del Collado M , Sangalli JR , Nociti RP , da Silva Junior WA , Bastien A , Robert C , Meirelles FV , Perecin F , da Silveira JC
Biol Reprod.2019 Sep 02 ; ():.
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Extracellular vesicles (EVs) are nanoparticles secreted by ovarian follicle cells. EVs are an important form of intercellular communication, since they carry bioactive contents, such as microRNAs, mRNAs, and proteins. MicroRNAs are small non-coding RNA capable of modulating mRNA translation. Thus, EVs can play a role in follicle and oocyte development. However, it is not clear if EV contents vary with the estrous cycle stage. The aim of this study was to investigate the bovine microRNA content in EVs obtained from follicles at different estrous cycle stages, which are associated with different progesterone (P4) levels in the follicular fluid (FF). We collected FF from 3-6 mm follicles and evaluated the microRNA profile of the EVs and their effects on cumulus-oocyte complexes (COCs) during in vitro maturation (IVM). We observed that EVs from low P4 group have a higher abundance of microRNAs predicted to modulate pathways, such as MAPK, RNA transport, Hippo, Cell cycle, FoxO, oocyte meiosis, and TGF-beta. Additionally, EVs were taken up by cumulus cells, and, thus, affected the RNA global profile 9 h after EV supplementation. Cumulus cells supplemented with EVs from low P4 presented up-regulated genes that could modulate biological processes, such as oocyte development, immune responses, and Notch signaling compared to genes of cumulus cells in the EV free media or with EVs from high P4 follicles. In conclusion, our results demonstrate that EV microRNA contents are distinct in follicles exposed to different estrous cycle stage. Supplementation with EVs impacts gene expression and biological processes in cumulus cells.
PMID: 31504242 [PubMed - as supplied by publisher]
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