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Spectroscopic approach for the interaction of carbon nanoparticles with cytochrome c and BY-2 cells: Protein structure and mitochondrial function.

著者 Chen L , Hao J , Xu L , Meng X , Li X , Nie C , Xie F , Liu K , Peng X , Xie J , Liang T , Guo Z
Int J Biol Macromol.2019 Jul 11 ; ():.
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In this study, we employed multiple spectroscopic methods to analyze the effects of carbon nanoparticles (CNPs) on structure of cytochrome c (Cyt c) and mitochondrial function in plant cells. The tertiary structures of aromatic amino acid in Cyt c were not changed after addition of CNPs. Cyt c was found to be absorbed on the surfaces of CNPs in a non-linear manner and only bound Cyt c can be reduced. In addition, the binding of Cyt c was found to increase the diameter of CNPs at lower concentrations. The redox potential of Cyt c was almost not affected after treatment with CNPs. There were no obvious differences in cellular ATP after exposure to CNPs, and the mitochondrial membrane potential (MMP) was significantly decreased once the CNPs concentration exceeded 31.25 μg/mL. The levels of reactive oxygen species (ROS) also were increased in BY-2 cells. Taken together, these findings provide basis for the interactions between CNPs and Cyt c, as well as the effect of CNPs treatment on the mitochondria function in plant cells.
PMID: 31302123 [PubMed - as supplied by publisher]
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