絞り込み

16634

広告

Engineering of Bioinspired, Size-controllable and Self-degradable Cancer-targeting DNA Nanoflowers via Incorporation of an Artificial Sandwich Base.

著者 Zhang L , Abdullah R , Hu X , Bai H , Fan H , He L , Liang H , Zou J , Liu Y , Sun Y , Zhang X , Tan W
J Am Chem Soc.2019 Feb 07 ; ():.
この記事をPubMed上で見るPubMedで表示
この記事をGoogle翻訳上で見る Google翻訳で開く

スターを付ける スターを付ける     (5view , 0users)

Full Text Sources

In this paper, we used an artificial DNA base to manipulate the formation of DNA nanoflowers (NFs) to easily control their sizes and functionalities. Nanoflowers have been reported as the noncanonical self-assembly of multifunctional DNA nanostructures, assembled from long DNA building blocks generated by rolling circle replication (RCR). They could be incorporated with myriad functional moieties. However, the efficacy of these DNA NFs as potential nanocarrier delivering cargoes in biomedicine is limited by the bioavailability and therapeutic efficacy of their cargo. Here we report the incorporation of metal-containing artificial analogues into DNA strands to control the size and the functions of NFs. We have engineered bioinspired, size-controllable and self-degradable cancer-targeting DNA nanoflowers (Sgc8-NFs-Fc) via incorporation of an artificial sandwich base. More specifically, the introduction of a ferrocene base not only resulted in size controllability of Sgc8-NFs-Fc from 1000 to 50 nm, but also endowed Sgc8-NFs-Fc with self-degradability in the presence of H2O2 via Fenton's reaction. In vitro experiments confirmed that Sgc8-NFs-Fc/Dox could be selectively uptaken by protein tyrosine kinase 7 (PTK7)-positive cancer cells and subsequently cleaved via Fenton's reaction, resulting in rapid release kinetics, nuclear accumulation, and enhanced cytotoxicity of their cargo. In vivo experiments further confirmed that Sgc8-NFs-Fc have good tumor-targeting ability and could significantly improve the therapeutic efficacy of Doxorubicin in a xenograft tumor model. Based on their tunable size and on-demand drug release kinetics upon H2O2 stimulation, the Sgc8-NFs-Fc nanocarriers possess promising potential in drug delivery.
PMID: 30730715 [PubMed - as supplied by publisher]
印刷用ページを開く Endnote用テキストダウンロード