絞り込み

16643

広告

ノーベル化学賞の吉野さん 理事長務める大阪の研究施設に出勤

ノーベル化学賞に選ばれた吉野彰さんが、受賞決定後初めて、自身が理事長を務める大阪府内のリチウムイオン電池の研究施設に出勤し、研究への変わらぬ意欲を示しました。

  1. [医学] ICU患者への控えめな酸素供給...
  2. 移植不適格な新規診断多発性骨髄腫へのD-...
  3. 移植不適格な新規診断多発性骨髄腫に対して...
  4. 「イクメン企業アワード2019」・「イク...

ニュース一覧

LC-MS/MS Method for Determination of Epothilone B in Rat Plasma and its Application in Pharmacokinetic Study.

著者 Lu HM , Ye M
Arzneimittelforschung.2012 Oct 23 ; ():.
この記事をPubMed上で見るPubMedで表示
この記事をGoogle翻訳上で見る Google翻訳で開く

Department of Oncology, Renji Hospital, Medical College, Shanghai Jiaotong University, Shanghai, China.

スターを付ける スターを付ける     (711view , 0users)

Full Text Sources

A simple LC-MS/MS method was developed for determination and pharmacokinetic study of Epothilone B in rat plasma.Plasma sample pretreatment involved a one-step liquid-liquid extraction of 100 µL plasma. The chromatographic separation was carried out on a Agilent Zobax SB C18 column with a mobile phase consisting of 10 mmol/L ammonium acetate-acetonitrile (35:65, v/v) at a flow rate of 0.2 mL/min. The detection was performed on a triple quadrupole tandem mass spectrometer by SRM via electro spray ionization source with positive mode.The standard curve for Epothilone B was linear over the concentration range of 1-100 ng/mL with a lower limit of quantification of 0.5 ng/mL. The intra- and inter-day precision (relative standard deviation) values were not higher than 15% and the accuracy (relative error) was < 10% at 3 quality control levels. Pharmacokinetic parameters were as follows: t1/2, 3.56 (1.12) h; AUC0-24 h, 295.7 (65.3) ng · h/mL and AUC0-∞, 339.2 (87.4) ng · h/mL, CL, 5.77 (0.67) mL/h; MRT, 7.55 (2.41) h, respectively.This simple, fast and highly sensitive method was fully validated and successfully applied to a preclinical pharmacokinetic study of Epothilone B in rats after i. v. administration.
PMID: 23093482 [PubMed - as supplied by publisher]
印刷用ページを開く Endnote用テキストダウンロード