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「"methods enzymol"[Journal]」の検索結果

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Preface.

Production of Human ATG Proteins for Lipidation Assays.

Use of pHlurorin-mKate2-human LC3 to Monitor Autophagic Responses.

High-Throughput Quantification of GFP-LC3(+) Dots by Automated Fluorescence Microscopy.

Turnover of Lipidated LC3 and Autophagic Cargoes in Mammalian Cells.

Assessing the Catalytic Activity of Transglutaminases in the Context of Autophagic Responses.

Allosteric Modulation of AMPK Enzymatic Activity: In Vitro Characterization.

Methods to Study Lysosomal AMPK Activation.

In Vitro Characterization of VPS34 Lipid Kinase Inhibition by Small Molecules.

The Use of DQ-BSA to Monitor the Turnover of Autophagy-Associated Cargo.

Methods to Study the BECN1 Interactome in the Course of Autophagic Responses.

Evaluating the mTOR Pathway in Physiological and Pharmacological Settings.

Study of ULK1 Catalytic Activity and Its Regulation.

In Vitro Reconstitution of Atg8 Conjugation and Deconjugation.

In Vitro Reconstitution of Autophagosome-Lysosome Fusion.

A Simple Cargo Sequestration Assay for Quantitative Measurement of Nonselective Autophagy in Cultured Cells.

Ultrastructural Characterization of Phagophores Using Electron Tomography on Cryoimmobilized and Freeze Substituted Samples.

Fluorescence-Based Assays to Analyse Phosphatidylinositol 5-Phosphate in Autophagy.

Mass Assays to Quantify Bioactive PtdIns3P and PtdIns5P During Autophagic Responses.

Quantification of Phosphatidylinositol Phosphate Species in Purified Membranes.

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