絞り込み

17703

広告

Platelet binding of IgG from patients with heparin-induced thrombocytopenia.

著者 Horne MK , Alkins BR
J Lab Clin Med.1996 May ; 127(5):435-42.
この記事をPubMed上で見るPubMedで表示
この記事をGoogle翻訳上で見る Google翻訳で開く

Clinical Pathology Department, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA.

スターを付ける スターを付ける     (53view , 0users)

Full Text Sources

Medical

Miscellaneous

Other Literature Sources

Although heparin induces immune-mediated thrombocytopenia, it has been difficult to demonstrate heparin specificity of the putative immunoglobin. Recently, however, a body of data has indicated that platelet factor 4 (PF4) is required for heparin-induced thrombocytopenia (HIT) antibody to bind to heparin. Using viable platelets in a physiologic buffer, we have now documented specific and reversible platelet binding of iodine 125-labeled IgG from 5 patients with HIT and binding of 125I-labeled F(ab')2 from 2 of them. The binding requires the presence of both heparin and PF4 in a molar ratio of approximately 1:1. We have also shown that platelet activation increases HIT antibody binding. Our data suggest that the F(ab')2 domain of HIT antibody binds to heparin-PF4 complexes that accumulate on the platelet surface when equimolar concentrations of heparin and PF4 are present.
PMID: 8621980 [PubMed - indexed for MEDLINE]
印刷用ページを開く Endnote用テキストダウンロード