絞り込み

16762

広告

院内トリアージ実施料の範囲を拡大へ (日経BP)

2020年4月8日、中央社会保険医療協議会(中医協)総会が開催され、新型コロナウイルス感染症に伴う医療保険制度の対応が議論された。 厚生労働省保険局医療課は、外...

  1. 「第4の携帯」楽天が船出 コロナで一部店...
  2. ペムブロリズマブのTMB高値既治療固形癌...
  3. 中等症の患者を集めて高次病院の負担軽減目...
  4. 令和2年3月末申請の収容定員の増加に係る...

ニュース一覧

Indole contributes to tetracycline resistance via the outer membrane protein OmpN in Vibrio splendidus.

著者 Zhang S , Shao Y , Zhao X , Li C , Guo M , Lv Z , Zhang W
World J Microbiol Biotechnol.2020 Feb 22 ; 36(3):36.
この記事をPubMed上で見るPubMedで表示
この記事をGoogle翻訳上で見る Google翻訳で開く

スターを付ける スターを付ける     (1view , 0users)

Full Text Sources

As an interspecies and interkingdom signaling molecule, indole has recently received attention for its diverse effects on the physiology of both bacteria and hosts. In this study, indole increased the tetracycline resistance of Vibrio splendidus. The minimal inhibitory concentration of tetracycline was 10 μg/mL, and the OD of V. splendidus decreased by 94.5% in the presence of 20 μg/mL tetracycline; however, the OD of V. splendidus with a mixture of 20 μg/mL tetracycline and 125 μM indole was 10- or 4.5-fold higher than that with only 20 μg/mL tetracycline at different time points. The percentage of cells resistant to 10 μg/mL tetracycline was 600-fold higher in the culture with an OD of approximately 2.0 (higher level of indole) than that in the culture with an OD of 0.5, which also meant that the level of indole was correlated to the tetracycline resistance of V. splendidus. Furthermore, one differentially expressed protein, which was identified as the outer membrane porin OmpN using SDS-PAGE combined with MALDI-TOF/TOF MS, was upregulated. Consequently, the expression of the ompN gene in the presence of either tetracycline or indole and simultaneously in the presence of indole and tetracycline was upregulated by 1.8-, 2.54-, and 6.01-fold, respectively, compared to the control samples. The combined results demonstrated that indole enhanced the tetracycline resistance of V. splendidus, and this resistance was probably due to upregulation of the outer membrane porin OmpN.
PMID: 32088790 [PubMed - as supplied by publisher]
印刷用ページを開く Endnote用テキストダウンロード