絞り込み

16645

広告

氷上のワカサギ釣り解禁 暖冬で10日遅れ 前橋 群馬

前橋市の、赤城山にある湖で冬の風物詩、氷上のワカサギ釣りが暖冬による影響で例年よりも10日ほど遅れて22日朝、解禁され多くの愛好家でにぎわっています。

  1. 新型ウイルス肺炎 死者9人 患者440人...
  2. 色々な色の名前、日本語と英語での名前、伝...
  3. 調達情報 企業啓発用ガイドブック201,...
  4. 調達情報 令和2年度~令和4年度診療報酬...

ニュース一覧

Yes-associated protein regulates podocyte cell cycle re-entry and dedifferentiation in adriamycin-induced nephropathy.

著者 Xie K , Xu C , Zhang M , Wang M , Min L , Qian C , Wang Q , Ni Z , Mou S , Dai H , Pang H , Gu L
Cell Death Dis.2019 Dec 04 ; 10(12):915.
この記事をPubMed上で見るPubMedで表示
この記事をGoogle翻訳上で見る Google翻訳で開く

スターを付ける スターを付ける     (17view , 0users)

Full Text Sources

Miscellaneous

Podocytes are terminally differentiated cells with little proliferative capacity. The high expression levels of cell cycle inhibitory proteins, including p21, p27, and p57, play an important role in maintaining the low level of proliferation of mature podocytes. In the present study, we aimed to explore the role of yes-associated protein (YAP) signalling in adriamycin-induced podocyte re-entry into the cell cycle and dedifferentiation. Proliferating cell nuclear antigen (PCNA)-, cyclin-dependent kinase 4 (CDK4)-, and Cyclin D1-positive podocytes were found in mice with adriamycin-induced nephropathy. In vitro, adriamycin administration increased the percentage of cells in S phase and the upregulation of mesenchymal-related marker proteins. CDK4 and cyclin D1 were significantly up-regulated after incubation with adriamycin. Overexpression of YAP in podocytes promoted their entry into the cell cycle; up-regulated cyclin D1, desmin, and snail2 expression and down-regulated Wilms' tumour 1 (WT1) and nephrin production. Recombinant murine FGF-basic induced podocytes to re-enter the cell cycle, inhibited WT1 and nephrin, and increased desmin and snail2 expression. Pretreating podocytes with verteporfin, an inhibitor of YAP/ TEA domain transcription factor (TEAD), decreased the adriamycin-induced overexpression of cyclin D1 and reduced the ratio of S-phase podocytes. This result was further verified by knocking down YAP expression using RNA interference. In conclusion, adriamycin induced podocytes to re-enter the cell cycle via upregulation of CDK4 and cyclin D1 expression, which was at least partly mediated by YAP signalling. Re-entry into the cell cycle induced the over-expression of mesenchymal markers in podocytes.
PMID: 31801948 [PubMed - in process]
印刷用ページを開く Endnote用テキストダウンロード