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Knockout of the Gene Improves the Stability of dsRNA and Increases the Sensitivity of to Toxin.

著者 Guan R , Chen Q , Li H , Hu S , Miao X , Wang G , Yang B
Front Physiol.2019 ; 10():1368.
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Double-stranded RNA (dsRNA)-induced genes are usually related to RNA interference (RNAi) mechanisms and are involved in immune-related pathways. In a previous study, we found a lepidopteran-specific nuclease gene that was up-regulated by dsRNA and that affected RNAi efficiency in Asian corn borer (). In this study, to verify the function of , the homologous gene in cotton bollworm () was knocked out using CRISPR/Cas9 system. We found that the midgut epithelium structure was apparently not affected in the Δ mutant [Knock out (KO)]. Transcript sequencing results showed that most of the known insect immune-related genes were up-regulated in KO. When second instar larvae were fed artificial diet with Cry1Ac, a protoxin from (), in sublethal doses (2.5 or 4 μg/g), the growth rate of KO was repressed significantly. The dsRNA stability was also enhanced in midgut extraction of KO; however, RNAi efficiency was not obviously improved compared with the wild type (WT). The KO and WT were injected with dsEGFP (Enhanced green fluorescent protein) and subjected to transcriptome sequencing. The results showed that the expression levels of 14 nuclease genes were enhanced in KO after the dsRNA treatment. These findings revealed that expression level was not only related with dsRNA stability, but also with resistance in cotton bollworm. When was knocked out, other immune- or nuclease-related genes were enhanced significantly. These results remind us that insect immune system is complex and pest control for cotton bollworm is an arduous task.
PMID: 31708803 [PubMed]
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