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Validation and kinetic of enzymatic method for the detection of organophosphate insecticides based on cholinesterase inhibition.

著者 Badawy SM
Toxicol Mech Methods.2019 Sep 18 ; ():1-14.
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Kinetic and validation of the enzymatic method for determination of fenitrothion organophosphorus based on cholinesterase inhibition were studied. A Linear relationship was obtained with a determination coefficient R of 0.9989 suggesting that the noncompetitive inhibition kinetic equation is suitable to represent the enzymatic assay of fenitrothion. The value of the inhibition constant was 0.374 ug/ml/min. The analytical logarithmic curve for the determination of fenitrothion concentration using the percentage of cholinesterase inhibition presented good linear relations at concentrations of 0.05 to 2 μg/mL (R = 0.9889). The maximum inhibition 83% was observed at 2.0 μg/mL final assay concentration. The lower inhibition 3.3% was observed at 0.05 μg/mL detection limit. The experimental measurement condition were optimized. The enzymatic method exhibited detection limits (LOD) in the range of 0.05 to 2.0 ug/ml. The limit of quantification (LOQ) was 0.06 μg/mL with inhibition 13%. The concentration of fenitrothion that inhibited the hydrolysis of substrate by 50% (IC50 value) was 0.4 μg/mL. Standard deviations and coefficients of variation indicate a good precision of the enzymatic method for the detection of organophosphate insecticides at an incubation time of 20 min.
PMID: 31532269 [PubMed - as supplied by publisher]
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