絞り込み

16643

広告

The C Ppc promoters of many C grass species share a common regulatory mechanism for gene expression in the mesophyll cell.

著者 Das Gupta S , Levey M , Schulze S , Karki S , Emmerling J , Streubel M , Gowik U , Quick WP , Westhoff P
Plant J.2019 Sep 17 ; ():.
この記事をPubMed上で見るPubMedで表示
この記事をGoogle翻訳上で見る Google翻訳で開く

スターを付ける スターを付ける     (2view , 0users)

Full Text Sources

Miscellaneous

C photosynthetic plants have evolved from C ancestors and are characterized by differential expression of several hundred genes. Strict compartmentalization of key C enzymes either to mesophyll (M) or bundle sheath (BS) cells is considered a crucial step towards the evolution of C photosynthesis. In this study we demonstrate that the 5´-flanking sequences of the C type phosphoenolpyruvate carboxylase (Ppc) gene from three C grass species could drive M cell-specific expression of a reporter gene in rice. In addition to that, we identified around 450 bp (upstream of their transcription start site) of the analyzed C Ppc promoters contain all the essential regulatory elements for driving M cell-specific expression in rice leaves. Importantly, four motifs of conserved nucleotide sequences (CNS)s were also determined, which are essential for the activity of the promoter. A putative interaction between the CNSs and an unknown upstream element(s) is required for driving M cell-specific expression. This work identifies the evolutionary conservation of C Ppc regulatory mechanisms of multiple closely related C grass species.
PMID: 31529521 [PubMed - as supplied by publisher]
印刷用ページを開く Endnote用テキストダウンロード