絞り込み

16532

広告

Fungicidal effect of chitosan via inducing membrane disturbance against Ceratocystis fimbriata.

著者 Xing K , Xing Y , Liu Y , Zhang Y , Shen X , Li X , Miao X , Feng Z , Peng X , Qin S
Carbohydr Polym.2018 Jul 15 ; 192():95-103.
この記事をPubMed上で見るPubMedで表示
この記事をGoogle翻訳上で見る Google翻訳で開く

スターを付ける スターを付ける     (33view , 0users)

Full Text Sources

Miscellaneous

In this study, the fungicidal effects and detailed action of chitosan against Ceratocystis fimbriata were evaluated. The results demonstrated that chitosan exhibited strong antifungal activity that restricted the mycelium extension and changed the hyphal morphology. Fluorescein diacetate (FDA) and propidium iodide (PI) double-staining directly visualized decreased cell viability in response to chitosan treatment. Investigation of the PI influx showed that chitosan induced irreversible cell membrane damage. The efflux of potassium ions from the cytosol into the extracellular matrix demonstrated that chitosan induced the leakage of intracellular components. Massive intracellular bis-(1, 3-dibutylbarbituric acid) trimethine oxonol [DiBAC(3)] accumulation indicated the dissipation of membrane potential. Furthermore, chitosan clearly decreased the activity of H/K ATPase. Fluorescence microscopy revealed that the fluorescence distribution and intensity of fluorescein isothiocyanate (FITC) changed as the incubation time increased. These results indicate that chitosan exerts a fungicidal effect via its ability to disturb fungal membranes.
PMID: 29691039 [PubMed - in process]
印刷用ページを開く Endnote用テキストダウンロード