絞り込み

16555

広告

J-Startupをはじめとするベンチャー企業の入札機会を拡大します

経済産業省は、関係府省庁と連携して、政府調達に関する統一基準を改訂しました。これにより、あらゆる規模の政府調達に対してJ-Startup企業の入札が可能となりま...

  1. FB、副社長に英の元副首相 規制強化のE...
  2. 選手は1型糖尿病 日本の患者励まし参戦 ...
  3. 日欧の水星探査機、打ち上げ成功…25年に...
  4. いつになったら我々は地球工学しか方法はな...

ニュース一覧

A Novel and Label-free Platform for MicroRNA Detection Based on the Fluorescence Quenching of Positively Charged Gold Nanoparticles to Ag Nanoclusters.

著者 Miao X , Cheng Z , Ma H , Li Z , Xue N , Wang P
Anal Chem.2017 Dec 04 ; ():.
この記事をPubMed上で見るPubMedで表示
この記事をGoogle翻訳上で見る Google翻訳で開く

スターを付ける スターを付ける     (38view , 0users)

Full Text Sources

A novel strategy was developed for microRNA-155 (miRNA-155) detection based on the fluorescence quenching of positively charged gold nanoparticles ((+)AuNPs) to Ag nanoclusters (AgNCs). In the designed system, DNA-stabilized Ag nanoclusters (DNA/AgNCs) were introduced as fluorescent probes, and DNA-RNA heteroduplexes were formed upon the addition of target miRNA-155. Meanwhile, the (+)AuNPs could be electrostatically adsorbed on the negatively charged single-stranded DNA (ssDNA) or DNA-RNA heteroduplexes to quench the fluorescence signal. In the presence of duplex-specific nuclease (DSN), DNA-RNA heteroduplexes became a substrate for the enzymatic hydrolysis of DNA strand to yield a fluorescence signal due to the diffusion of AgNCs away from (+)AuNPs. Under the optimal conditions, (+)AuNPs displayed 95% of the quenching efficiency to AgNCs, which paved the way for ultrasensitive detection with a low detection limit of 33.4 fM. In particular, the present strategy demonstrated excellent specificity and selectivity toward the detection of target miRNA against control miRNAs, including mutated miRNA-155, miRNA-21, miRNA-141, let-7a, and miRNA-182. Moreover, the practical application value of the system was confirmed by the evaluation of the expression levels of miRNA-155 in clinical serum samples with satisfactory results, suggesting that the proposed sensing platform is promising for applications in disease diagnosis as well as the fundamental research of biochemistry.
PMID: 29198110 [PubMed - as supplied by publisher]
印刷用ページを開く Endnote用テキストダウンロード