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原子炉建屋想定し高専生技術競う (デイリースポーツ)

東京電力福島第1原発の原子炉建屋を想定した会場で、高専生が自作ロボットの操作技術を競う「廃炉創造ロボコン」が16日、福島県楢葉町で開かれた。廃炉技術に興味を持っ...

  1. [企業] Andreessen Horo...
  2. 金井さん、17日宇宙へ (デイリースポー...
  3. 宇宙飛行士の金井さん あす打ち上げ 長期...
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Individual evaluation of cardiac marker expression and self-beating during cardiac differentiation of P19CL6 cells on different culture substrates.

著者 Yamaoka T , Hirata M , Dan T , Yamashita A , Otaka A , Nakaoki T , Miskon A , Kakinoki S , Mahara A
J Biomed Mater Res A.2016 Dec 15 ; ():.
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Cell-based therapies using self-beating cardiomyocytes have been attracting great attention for use in cardiac regeneration, although an effective procedure to improve cardiac differentiation and self-beating induction is required. The purpose of this study is to clarify the effect of the culture substrate on cardiac maturation by separately evaluating the cardiac differentiation step and the beating induction step in vitro. To this end, the well-studied cardiomyocyte-like progenitor cell line P19CL6 and neonatal cardiomyocytes (NCMs) were selected and cultured on substrates coated with collagen type I (Col-I), gelatin (Gel), fibronectin (FN), or poly-L-lysine (PLL). It was found that the cardiac differentiation step, which was assessed using cardiac marker gene expression (GATA-binding protein 4 (GATA4), myocyte-specific enhancer factor 2D (MEF2D), and hyperpolarization-activated cyclic nucleotide-gated potassium channel 4 (HCN4)) in the P19CL6 embryonal carcinoma cells, was greatly enhanced on Col-I, Gel, and PLL. In contrast, the spontaneous beating step, which was directly assessed by counting the beating colonies and measuring contractile protein gene expression (α-myosin heavy chain (α-MHC), troponin C type 1 (TnC1), and troponin T type 2 (TnT2)) in the rat NCMs, was enhanced on the FN and PLL surfaces. In the present study, for the first time, it was found that PLL enhances both the cardiac differentiation and the beating induction steps of cardiac maturation, which can aid in preparing beating cardiomyocytes for regenerative medicine. This article is protected by copyright. All rights reserved.
PMID: 27977899 [PubMed - as supplied by publisher]
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