絞り込み

16404

広告

目標上積みの「タラノア対話」合意 (毎日新聞)

[PR] 温室ガス削減 「パリ協定」控え18年の1年間かけ実施へ 【ボン五十嵐和大】ドイツのボンで開催中の国連気候変動枠組み条約第23回締約国会議(COP23)...

  1. ドイツの温暖化会議で決議採択 - 福井新...
  2. 国内初の種類 恐竜の卵の化石を展示 山口...
  3. 温暖化、COP23大筋合意 先進国の削減...
  4. 本人望めば蘇生中止 消防庁委託研究班提言...

ニュース一覧

Specific recognition of biologically active amyloid-β oligomers by a new Surface Plasmon Resonance-based immunoassay and an in vivo assay in Caenorhabditis elegans.

著者 Stravalaci M , Bastone A , Beeg M , Cagnotto A , Colombo L , Di Fede G , Tagliavini F , Cantu' L , Del Favero E , Mazzanti M , Chiesa R , Salmona M , Diomede L , Gobbi M
J Biol Chem.2012 Jun 26 ; ():.
この記事をPubMed上で見るPubMedで表示
この記事をGoogle翻訳上で見る Google翻訳で開く

Istituto di Ricerche Farmacologiche Mario Negri, Italy;

スターを付ける スターを付ける     (330view , 0users)

Full Text Sources

Other Literature Sources

Soluble oligomers of the amyloid-β (Aβ) peptide play a key role in the pathogenesis of Alzheimer's disease, but their elusive nature makes their detection challenging. Here we describe a novel immunoassay, based on surface plasmon resonance (SPR), that specifically recognizes biologically active Aβ oligomers. As capturing agent, we immobilized on the sensor chip the monoclonal antibody 4G8, which targets a central hydrophobic region of Aβ. This SPR assay allows to specifically recognize oligomeric intermediates which rapidly appear and disappear during the incubation of synthetic Aβ1-42, discriminating them from monomers and higher-order aggregates. The species recognized by SPR generate ionic currents in artificial lipid bilayers and inhibit the physiological pharyngeal contractions in C.elegans, a new method for testing the toxic potential of Aβ oligomers. With these assays we found that the formation of biologically relevant Aβ oligomers is inhibited by epigallocatechin gallate and increased by the A2V mutation, previously reported to induce early-onset dementia. The SPR-based immunoassay provides new opportunities for detection of toxic Aβ oligomers in biological samples and could be adapted to study misfolding proteins in other neurodegenerative disorders.
PMID: 22736768 [PubMed - as supplied by publisher]
印刷用ページを開く Endnote用テキストダウンロード